reverse primer造句
例句與造句
- clone human wnk4 full length cdna into pgem-t vector human kidney total una was extracted and used as template to amplify wnk4 full length cdna with the forward primer ( 7-27 ) and reverse primer ( 3808-3833 ) with the long template expanded pcr system kit
5.原位雜交以小鼠腎臟總翩a為模板，擴(kuò)增wnki基因外顯子1，24一28，以a片段和wnk4基因外顯子13一16片段，純化后連接在pgem一t載體上。 - then, a piece of degenerate primer was designed according to the conserved amino acids of glycine betaine abc transporter system glycine betaine-binding protein as a reversed primer . combined with the opuaa-up, a 2.3 kb fragment was obtained through pcr . blast result showed a fragment which contained the partial opuaa, the whole opuab and partial opuac sequences were obtained
再次，根據(jù)甘氨酸甜菜堿atp轉(zhuǎn)運(yùn)系統(tǒng)底物結(jié)合蛋白的氨基酸保守序列設(shè)計(jì)下游簡(jiǎn)并引物，與atp結(jié)合蛋白的上游簡(jiǎn)并引物組合，經(jīng)pcr擴(kuò)增獲得2.1kb的條帶，測(cè)序后通過(guò)blast比較，結(jié)果顯示獲得atp結(jié)合蛋白基因的部分序列、通透酶的全部編碼序列和部分甘氨酸甜菜堿結(jié)合蛋白基因的核苷酸序列。 - rt-pcr kit was from takara technology co . ltd . the sequence of the primer of pkb are shown below, together with all the sodn and saodn oligodeoxynucleotide were synthesized in takara technology co . ltd . pkb primers; forward primer 5'cga gaa gcc gcg acc caa cac3'and reverse primer 5atg cac tac cgc cgg a3 "
有絲分裂前，cdcz/cy山b、組蛋白h;激酶的活性通過(guò)被weel和myt激酶磷酸化cdcz的1卜巧和thr14殘基而抑制。在有絲分裂期mpf可以被cdc25活化，這兩個(gè)位點(diǎn)被去磷酸化，cdc25是蛋白水解酶，可以使cdcz的t打巧脫離磷酸化。 - It's difficult to find reverse primer in a sentence. 用reverse primer造句挺難的